酿酒葡萄香宝馨与赤霞珠正反交后代果实性状遗传变异分析.pdf
《酿酒葡萄香宝馨与赤霞珠正反交后代果实性状遗传变异分析.pdf》由会员分享,可在线阅读,更多相关《酿酒葡萄香宝馨与赤霞珠正反交后代果实性状遗传变异分析.pdf(14页珍藏版)》请在咨信网上搜索。
1、果树学报2023,40(8):1509-1522Journal of Fruit ScienceDOI:10.13925/ki.gsxb.20230019酿酒葡萄香宝馨与赤霞珠正反交后代果实性状遗传变异分析谢军1,2,王荣2,徐美隆3,乔改霞2,刘玉娟2,余泽龙2,李毅1*(1甘肃农业大学林学院,兰州 730070;2林木资源高效生产全国重点实验室,银川 750004;3宁夏农林科学院园艺研究所,银川 750002)摘要:【目的】探究酿酒葡萄果实性状的遗传规律。【方法】以香宝馨(Chambourcin)和赤霞珠(Cabernet Sauvignon)以及它们的正反交后代为试材,对其果粒质量、大
2、小等果实性状进行遗传变异和遗传倾向分析,并初筛选出表现优良的单株。【结果】正反交后代果实性状变异较为广泛;单粒质量、果粒横纵径、果形指数、果皮厚度以及种子数在正反交后代中的遗传呈正态或偏正态分布,符合数量性状的遗传特点;单粒质量、果粒横纵径、果形指数、果皮厚度以及种子数的平均值均小于亲本中亲值,表现出趋小的遗传倾向;单粒质量、果粒横径、种子数都整体表现出趋中偏低的遗传倾向,果粒纵径、果形指数表现出明显偏低遗传的倾向,果皮厚度表现出明显的趋中遗传倾向,果粒形状、果皮颜色、果皮涩味、果肉香味遗传倾向于亲本,果肉质地遗传倾向趋向于亲本赤霞珠,果肉颜色遗传倾向则趋向于亲本香宝馨。【结论】通过聚类分析结
3、合田间观察初选出表现优良的杂交单株XC090、XC120、XC215、XC252、CX032、CX070、CX073、CX095作为重点观察对象。关键词:酿酒葡萄;正反交后代;果实性状;遗传倾向中图分类号:S663.1文献标志码:A文章编号:1009-9980(2023)08-1509-14收稿日期:2023-01-30接受日期:2023-03-30基金项目:宁夏回族自治区自然科学基金项目(2022AAC03440)作者简介:谢军,男,工程师,博士,研究方向为葡萄种质资源收集保存与创新。Tel:13995405011,E-mail:*通信作者Author for correspondence.
4、Tel:13919085362,E-mail:Analysis of genetic tendency of fruit traits in the reciprocal hybrids ofwine grape Chambourcin and Cabernet SauvignonXIE Jun1,2,WANG Rong2,XU Meilong3,QIAO Gaixia2,LIU Yujuan2,YU Zelong2,LI Yi1*(1Forestry College,Gansu Agriculture University,Lanzhou 730070,Gansu,China;2State
5、Key Laboratory of Efficient Production of For-est Resources,Yinchuan 750004,Ningxia,China;3Institute of Horticulture,Ningxia Academy of Agricultural and Forestry Sciences,Yin-chuan 750002,Ningxia,China)Abstract:【Objective】The study aimed to investigate the genetic rules of fruit traits of wine grape
6、s toprovide a reference for the rational selection of parent varieties for hybridization and the prediction ofrepresentative types after hybridization and improve the competitiveness of China s wine products.【Methods】In the experiment,Chambourcin and Cabernet Sauvignon were used as parents,and 105 a
7、nd75 individuals with stable flowering and fruiting characters were selected from the reciprocal hybrids re-spectively.The fruit weight,fruit size,peel thickness,fruit shape,peel color and other fruit traits wereinvestigated and measured.The genetic variation and genetic tendency of fruit traits wer
8、e analyzed,andthe hybrid plants with excellent comprehensive performance were selected by cluster analysis combinedwith field observation.【Results】The reciprocal F1hybrids differed significantly in the berry weight,length,width,shape index,skin thickness,and seed number,and the data were close to a
9、normal orskewed normal distribution,exhibited genetic characteristics of quantitative traits.In particular,themean values of berry mass,berry length and width,berry shape index,berry skin thickness,and seednumber in offsprings were all lower than the median values of those of the parents,indicating
10、a genetic果树学报第40卷葡萄是世界上最重要的水果之一,栽培历史悠久,种植面积广泛,具有极高的经济效益和社会效益,因此在世界果品产业中占据着重要地位1-2。近些年来,中国葡萄与葡萄酒产业迅速发展,中国已经成为世界上的葡萄与葡萄酒生产大国,葡萄也成为中国主要栽培的果树树种之一3。但目前中国主栽的酿酒葡萄品种大多数为国外引进的品种,缺少具有较高影响力且能适应中国风土的自育品种4。另外,从前人研究报道中可以了解到,中国葡萄育种以鲜食选育为主,酿酒葡萄育种没有得到足够有效的重视5-7。因此,加大对酿酒葡萄育种的重视程度,开展选育具有自主知识产权且具有中国本土特色风味的酿酒葡萄新品种,对提高中国
11、葡萄酒产品市场竞争力以及促进中国葡萄与葡萄酒产业的持续发展意义重大8。国内外对葡萄杂交后代果实性状的相关研究已有报道,Song等9对2个西班牙葡萄(GracianoTem-pranillo)亲本及F1代分析表明,F1代的农艺性状和果实品质呈现出连续变异;刘政海等3对酿酒葡萄黑比诺与马瑟兰杂交后代果实性状遗传倾向分析表tendency to low,which might be related to the strong non-additive effect of genes.The coefficient varia-tion of berry weight was about 22.0%,
12、the heterosis rate was-11.96%and-10.53%,and the heritabilitywas 88.04%and 89.47%,respectively.The degree of variation was large,they had high genetic diversi-ty,and the heterosis rate and heritability were low compared with the other traits,which might be easilyaffected by environmental factors.The
13、coefficient variation of berry width and length ranged from 7.8to 8.7,which was relatively small,and the heritability was relatively high.The average peel thicknessof reciprocal cross was 0.29 mm,which was close to the mid-parent value.The coefficient of variationwas 11.37%and 12.64%,respectively,an
14、d the heritability was 96.03%and 95.99%,respectively,indi-cating that it could be stably inherited and was less affected by the environment.The coefficient varia-tion of berry shape index of the reciprocal cross was 3.29%and 3.88%,respectively,which was lowerthan those of the other traits.The hetero
15、sis rate and heritability were higher than those of the othertraits,indicating that the fruit shape index could be stably inherited and less affected by the environ-ment.The coefficient variation of the seed number of reciprocal cross was 20.83%and 20.16%,respec-tively,and the heritability was 92.38
16、%and 93.71%,respectively.The result suggested that the seednumber could be stably inherited but the degree of variation be relatively large.The berry weight,widthand seed number all showed a genetic tendency towards moderate to low as a whole;the vertical berrydiameter and fruit shape index showed a
17、 significant tendency to low;the peel thickness showed a signif-icant tendency to moderate;the berry shape,skin color,astringency,and aroma were all similar to theparents,and the flesh texture was heavily biased towards the parent Cabernet Sauvignon,while theflesh color was more influenced by the pa
18、rent Chambourcin.When Cabernet Sauvignon was used as thefemale parent and Chambourcin was used as the male parent,the probability of individuals with largefruit and fruit weight was greater than that of orthogonal.By cluster analysis,the orthogonal offspringscould be divided into two categories,the
19、first category included the parent Chambourcin and 32 off-springs,the second category included the parent Cabernet Sauvignon and 73 offsprings;the reversecross offspring could be divided into three categories,the first category included 32 offsprings,the sec-ond category included the father Chambour
20、cin and 4 offsprings,and the third category included themother Cabernet Sauvignon and 40 offsprings.It was clear that the fruit traits of the reciprocal cross off-springs were greatly affected by the parent Cabernet Sauvignon.【Conclusion】The fruit traits of the re-ciprocal hybrids of Chambourcin and
21、 Cabernet Sauvignon showed a genetic tendency to low and medi-um as a whole,and through clustering analysis combined with field observation,XC090,XC120,XC215,XC252,CX060,CX07,CX079,and CX090 were selected.Key words:Wine grape;Reciprocal hybrids;Fruit traits;Genetic tendency1510,等:果实性状遗传变异分析第8期明,F1代果
22、实性状均为数量性状,广泛分离呈连续分布;刘政海等4对威代尔与霞多丽葡萄杂交F1代果实性状的遗传倾向进行分析,发现杂交后代平均果粒质量呈趋小方向回归,酸糖含量呈现较广的分离且有趋向于高酸和低糖亲本的遗传趋势;Ban等10对葡萄一个杂交群体的98个个体的8个果实性状进行QTL分析,发现了一个与果粒质量相关的等位基因。这些结果将为葡萄选择育种提供一定的理论支持。另外,目前常用的果实性状评价方法有感官评定、方差分析、相关性分析、聚类分析以及主成分分析等。郭瑶等11以山葡萄双红和欧亚种葡萄贵人香的杂种F1代为试材,采用层次分析法对F1代的果实特性、果穗特性及其他特性三方面进行了综合性的评价,从杂交后代中
23、选出了2个单株作为重点选育对象;王小龙等12以华葡1号和美乐杂交后代的12个株系成熟果实为材料,并采用主成分分析法对所测的15个指标进行了综合评价,从中筛选出一个综合表现最佳的单株;谭伟等8以酿酒葡萄亲本及杂交后代共74份成熟期果实为材料,并对其22个果实品质性状进行主成分分析和聚类分析,筛选出综合性状优良的杂交单系2份,并认为主成分分析和聚类分析可以简化酿酒葡萄杂交后代果实品质的评价指标。笔者在本研究中以酿酒葡萄香宝馨(CabernetSauvignon)和赤霞珠(Chambourcin)以及它们的正反交后代为研究对象,对其果粒形状、颜色等表型性状以及果粒质量、果粒大小等数量性状进行遗传变异
24、和遗传倾向分析,以期为酿酒葡萄杂交育种中亲本的合理选配及杂交后代表型的预测提供参考依据。1材料和方法1.1材料以赤霞珠(编号:CS)和香宝馨(编号:CH)为亲本于2016年进行杂交。赤霞珠属欧亚种,原产于法国波尔多地区,是世界上最著名的优质红色酿酒葡萄品种,有研究表明赤霞珠是由品丽珠与长相思自然杂交形成的13。香宝馨属欧美杂交种,原产于法国,由 Seyve Villard 12-417 与 Seibel 7053 杂交而成14。以“CHCS”(编号:XC)为正交,以“CSCH”(编号:CX)为反交,杂交后代于2018年定植于宁夏林业研究院股份有限公司葡萄种质资源圃,株行距为0.5 m2.5 m
25、,土质为沙土,栽培以及田间管理水平一致。经过分子标记鉴定和3 a(年)的形态与生物学特性的调查,2022年从正反交后代中分别筛选出能稳定开花结果的105株、75株个体以及其亲本作为试验材料,当种子变褐时进行果实基本品质性状的调查。1.2方法参考 葡萄种质资源描述规范和数据标准15,对葡萄果粒质量等数量性状和部分表型描述性状(表1)进行调查。亲本及杂交后代的果实成熟后,每个单株随机选取15个果粒,分别测定果粒质量、果粒横径、果粒纵径、果皮厚度、种子数,部分描述性状由专业人员参与测定。果粒质量用百分之一天平测定,果粒纵、横径用数显游标卡尺测量,果皮厚度用电子数显千分尺测量,记录种子数,果形指数用果
- 配套讲稿:
如PPT文件的首页显示word图标,表示该PPT已包含配套word讲稿。双击word图标可打开word文档。
- 特殊限制:
部分文档作品中含有的国旗、国徽等图片,仅作为作品整体效果示例展示,禁止商用。设计者仅对作品中独创性部分享有著作权。
- 关 键 词:
- 酿酒 葡萄 香宝馨 赤霞珠 正反交 后代 果实 性状 遗传 变异 分析
1、咨信平台为文档C2C交易模式,即用户上传的文档直接被用户下载,收益归上传人(含作者)所有;本站仅是提供信息存储空间和展示预览,仅对用户上传内容的表现方式做保护处理,对上载内容不做任何修改或编辑。所展示的作品文档包括内容和图片全部来源于网络用户和作者上传投稿,我们不确定上传用户享有完全著作权,根据《信息网络传播权保护条例》,如果侵犯了您的版权、权益或隐私,请联系我们,核实后会尽快下架及时删除,并可随时和客服了解处理情况,尊重保护知识产权我们共同努力。
2、文档的总页数、文档格式和文档大小以系统显示为准(内容中显示的页数不一定正确),网站客服只以系统显示的页数、文件格式、文档大小作为仲裁依据,平台无法对文档的真实性、完整性、权威性、准确性、专业性及其观点立场做任何保证或承诺,下载前须认真查看,确认无误后再购买,务必慎重购买;若有违法违纪将进行移交司法处理,若涉侵权平台将进行基本处罚并下架。
3、本站所有内容均由用户上传,付费前请自行鉴别,如您付费,意味着您已接受本站规则且自行承担风险,本站不进行额外附加服务,虚拟产品一经售出概不退款(未进行购买下载可退充值款),文档一经付费(服务费)、不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
4、如你看到网页展示的文档有www.zixin.com.cn水印,是因预览和防盗链等技术需要对页面进行转换压缩成图而已,我们并不对上传的文档进行任何编辑或修改,文档下载后都不会有水印标识(原文档上传前个别存留的除外),下载后原文更清晰;试题试卷类文档,如果标题没有明确说明有答案则都视为没有答案,请知晓;PPT和DOC文档可被视为“模板”,允许上传人保留章节、目录结构的情况下删减部份的内容;PDF文档不管是原文档转换或图片扫描而得,本站不作要求视为允许,下载前自行私信或留言给上传者【自信****多点】。
5、本文档所展示的图片、画像、字体、音乐的版权可能需版权方额外授权,请谨慎使用;网站提供的党政主题相关内容(国旗、国徽、党徽--等)目的在于配合国家政策宣传,仅限个人学习分享使用,禁止用于任何广告和商用目的。
6、文档遇到问题,请及时私信或留言给本站上传会员【自信****多点】,需本站解决可联系【 微信客服】、【 QQ客服】,若有其他问题请点击或扫码反馈【 服务填表】;文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“【 版权申诉】”(推荐),意见反馈和侵权处理邮箱:1219186828@qq.com;也可以拔打客服电话:4008-655-100;投诉/维权电话:4009-655-100。