云南省文山地区木腐菌真菌资源多样性研究(英文)_董军红.pdf
《云南省文山地区木腐菌真菌资源多样性研究(英文)_董军红.pdf》由会员分享,可在线阅读,更多相关《云南省文山地区木腐菌真菌资源多样性研究(英文)_董军红.pdf(25页珍藏版)》请在咨信网上搜索。
1、Research paper 研究论文 22 March 2023,42(3):638-662 菌物学报 Mycosystema ISSN1672-6472 CN11-5180/Q Doi:10.13346/j.mycosystema.220205 资助项目:国家自然科学基金(32170004)This work was supported by the National Natural Science Foundation of China(32170004).*Corresponding author.E-mail: ORCID:ZHAO Changlin(0000-0002-9653-7
2、018),DONG Junhong(0000-0001-8740-0805),GU Jinying(0000-0001-7653-4699)Received:2022-06-06;Accepted:2022-07-06 菌物学报 Copyright 2023 Institute of Microbiology,CAS.All rights reserved.| Http:/journals- Tel:+86-10-64807521 638 云南省文山地区木腐菌真菌资源多样性研究 董军红,顾金莹,赵长林*西南林业大学生物多样性保护学院,云南 昆明 650224 摘 要:本研究于 20172021
3、 年在云南省文山地区进行了 5 次实地调查,共采集木腐真菌标本2 923 号。采用经典分类学方法开展了该地区采集标本的准确鉴定,并编制了木腐真菌完整名录。采用最大似然法、最大简约法和贝叶斯推理法对研究标本的ITS nrRNA基因序列构建系统发育树,剖析分类单元的聚类关系及物种间的系统发育关系。研究结果揭示该地区共报道木腐真菌 104 种,隶属于 5 目 18 科 55 属。其中多孔菌目为优势目(占总数的 51.92%),多孔菌科为优势科(占总数的24.04%),木齿菌属为优势属(占总数的 12.50%)。关键词:担子菌门;生物资源;多样性;分子系统学;文山地区 引用本文 董军红,顾金莹,赵长林
4、,2023.云南省文山地区木腐菌真菌资源多样性研究.菌物学报,42(3):638-662 Dong JH,Gu JY,Zhao CL,2023.Diversity of wood-decaying fungi in Wenshan Area,Yunnan Province,China.Mycosystema,42(3):638-662 Diversity of wood-decaying fungi in Wenshan Area,Yunnan Province,China DONG Junhong,GU Jinying,ZHAO Changlin*College of Biodiversit
5、y Conservation,Southwest Forestry University,Kunming 650224,Yunnan,China Abstract:Five field surveys were carried out in Wenshan area,Yunnan Province,China during 20172021,and 2 923 specimens of wood-decaying fungi were collected.A checklist of wood-decaying fungi with their hosts and substrates is
6、given.The taxa were identified in the light of their morphology and molecular evidence.The sequences of ITS nrRNA gene region of the studied specimens were generated and the phylogenetic analyses were performed with the maximum likelihood,maximum parsimony and Bayesian inference methods.The paper su
7、mmarizes the obtained results of investigation on the wood-decaying fungi of this area,Research paper 22 March 2023,42(3):638-662 Mycosystema ISSN1672-6472 CN11-5180/Q 菌物学报 639 consisting of 104 species belonging to 55 genera,18 families and 5 orders.Polyporales is the dominant order(51.92%of the to
8、tal orders found in this area),and Polyporaceae is the dominant family(24.04%of the total)and Xylodon is the dominant genus(12.50%of the total).Keywords:Basidiomycota;biological resources;diversity;molecular systematics;Wenshan INTRODUCTION Fungi are a diverse,monophyletic group of eukaryotes and th
9、ese organisms show immense ecological and economic impacts for playing an important role in the ecosystems as diverse as soil,trees,hidden layers within their substrate(James et al.2020).Approximately 150 thousand species of fungi have been described(Dai et al.2021;Wang&Zhao 2021),but the potential
10、biodiversity of the group is likely to be 2.23.8 millions of species(Blackwell 2011;Taylor et al.2014;Hibbett 2016;Hawksworth&Lcking 2017).The diversity for flora of seed plants in Yunnan Province is higher than that in other areas of China.The number of discovered new fungal species totalled 1 345
11、from this province from 2000 to 2020.Endemic woody plants are rich in Yunnan,supplying rich and varied substrates for wood-decaying fungi.Wood-decaying fungi with industrial,medicinal,edible and economic value,comprise most basidiomycetes and ascomycetes growing on various kinds of living trees,dead
12、 and fallen trunk,fallen branch and stump(Russell&Paterson 2006;Dai 2012a;Dai et al.2015,2021;Vinay et al.2015;Wu et al.2019,2020;MBarek et al.2020;Runnel et al.2021;Luo et al.2022),displaying a considerable ability to degrade plant remains and different environmental contaminants through their exte
13、nsive organic compound degradation abilities(James et al.2020).The Wenshan area is located in the southeast part of Yunnan Province,including Laojunshan National Nature Reserve and Xiaoqiaogou National Nature Reserve and their surrounding areas(Yang et al.2008).The geographical location is between 1
14、034810452 E and 23162229 N with the altitude of 1 2002 991 m;the average annual precipitation is more than 1 500 mm(Yang et al.2008;Li et al.2021).The plant resources are rich,with 187 families,946 genera and 3 085 species of seed plants(Yang et al.2008).The vegetation types varied with elevation di
15、fferences(Yang et al.2008;Li et al.2021).The Wenshan area has five main types of well-preserved vegetation namely the mid-mountain wet,semi-wet evergreen broad-leaved forest,monsoon evergreen broad-leaved forest,mossy evergreen broad-leaved forest and mossy dwarf forest(Yang et al.2008),which are pr
16、econdition of the fungal diversity of this area.Most of wood-decaying fungi are reported in northwest Yunnan,and some polypore and the corticioid fungi have been reported in the southeast of the Yunnan Province(Xie 1992;Chen 1994;Zhang et al.2012;Guo et al.2013;Dai et al.2014;Chen et al.2020;Wang&Zh
17、ao 2021).According to the modern taxonomy,wood-decaying fungi mainly belong to ten orders of Agaricomycetes,viz.,Agaricales,Auriculariales,Cantharellales,Corticiales,Gloeophyllales,Hymenochaetales,Polyporales,Russulales,Thelephorales and Trechisporales(Dai 2012a;Dai et al.2015,2021;Wu et al.2020).Th
18、e current wood-decaying fungal catalogues include the poroid and corticioid hymenophores.In the present study,five field trips were carried out in Wenshan area,and 104 species were identified.This work aims at providing an updated checklist of wood-decaying fungi in Wenshan,as well as enriching the
19、knowledge of the fungal diversity of China.1 MATERIALS AND METHODS 1.1 Sample collection and herbarium specimen preparation The fresh fruiting bodies of basidiomycetous macrofungi growing on angiosperm stumps,trunks and branches were collected in 5 sampling points(approximately the four corners of t
20、he square and centre)within 11 km2 for 23 times every year in Wenshan area from 20172021.Three basidiomata DONG Junhong et al./Diversity of wood-decaying fungi in Wenshan Area,Yunnan Province,China 研究论文 菌物学报 640 at least are required and each individual is up to 3 cm long,2 cm wide,in which the smal
21、lest fruiting structure has to include the hymenophore.The samples were photographed in situ and its fresh macroscopic details were recorded.Photographs were recorded by a Jianeng 80D camera.All the photos were focus stacked and merged using Helicon Focus software.The samples were transported to a f
22、ield station where the fruit bodies were dried on an electronic food dryer(Fsfruit)at 45 C for 48 hours.Then the dried specimens were sealed in an envelope and zip-lock plastic bags and labeled.The dried specimens were deposited in the herbarium of Southwest Forestry University(SWFC),Kunming,Yunnan
23、Province,China.1.2 Morphological studies The macromorphological descriptions were based on field notes.The color terms are from those of Petersen(1996).The micromorphological data were obtained from the dried specimens and observed under a light microscope following Dai(2012a).The following abbrevia
24、tions were used for the micro-characteristics description:KOH,5%potassium hydroxide;CB,Cotton Blue;CB,acyanophilous;CB+,cyanophilous;IKI,Melzers reagent;IKI,both inamyloid and indextrinoid;L,mean spore length(arithmetic average of 30 spores);W,mean spore width(arithmetic average of 30 spores);Q,vari
25、ation in the L/W rationes between the specimens studied;n(a/b),number of spores(a)measured from given number(b)of specimens.1.3 Molecular procedures and phylogenetic analysis CTAB rapid plant genome extraction kit-DN14(Aidlab Biotechnologies Co.,Ltd)was used to obtain genomic DNA from dried specimen
- 配套讲稿:
如PPT文件的首页显示word图标,表示该PPT已包含配套word讲稿。双击word图标可打开word文档。
- 特殊限制:
部分文档作品中含有的国旗、国徽等图片,仅作为作品整体效果示例展示,禁止商用。设计者仅对作品中独创性部分享有著作权。
- 关 键 词:
- 云南省 文山 地区 木腐菌 真菌 资源 多样性 研究 英文 董军红
1、咨信平台为文档C2C交易模式,即用户上传的文档直接被用户下载,收益归上传人(含作者)所有;本站仅是提供信息存储空间和展示预览,仅对用户上传内容的表现方式做保护处理,对上载内容不做任何修改或编辑。所展示的作品文档包括内容和图片全部来源于网络用户和作者上传投稿,我们不确定上传用户享有完全著作权,根据《信息网络传播权保护条例》,如果侵犯了您的版权、权益或隐私,请联系我们,核实后会尽快下架及时删除,并可随时和客服了解处理情况,尊重保护知识产权我们共同努力。
2、文档的总页数、文档格式和文档大小以系统显示为准(内容中显示的页数不一定正确),网站客服只以系统显示的页数、文件格式、文档大小作为仲裁依据,平台无法对文档的真实性、完整性、权威性、准确性、专业性及其观点立场做任何保证或承诺,下载前须认真查看,确认无误后再购买,务必慎重购买;若有违法违纪将进行移交司法处理,若涉侵权平台将进行基本处罚并下架。
3、本站所有内容均由用户上传,付费前请自行鉴别,如您付费,意味着您已接受本站规则且自行承担风险,本站不进行额外附加服务,虚拟产品一经售出概不退款(未进行购买下载可退充值款),文档一经付费(服务费)、不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
4、如你看到网页展示的文档有www.zixin.com.cn水印,是因预览和防盗链等技术需要对页面进行转换压缩成图而已,我们并不对上传的文档进行任何编辑或修改,文档下载后都不会有水印标识(原文档上传前个别存留的除外),下载后原文更清晰;试题试卷类文档,如果标题没有明确说明有答案则都视为没有答案,请知晓;PPT和DOC文档可被视为“模板”,允许上传人保留章节、目录结构的情况下删减部份的内容;PDF文档不管是原文档转换或图片扫描而得,本站不作要求视为允许,下载前自行私信或留言给上传者【自信****多点】。
5、本文档所展示的图片、画像、字体、音乐的版权可能需版权方额外授权,请谨慎使用;网站提供的党政主题相关内容(国旗、国徽、党徽--等)目的在于配合国家政策宣传,仅限个人学习分享使用,禁止用于任何广告和商用目的。
6、文档遇到问题,请及时私信或留言给本站上传会员【自信****多点】,需本站解决可联系【 微信客服】、【 QQ客服】,若有其他问题请点击或扫码反馈【 服务填表】;文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“【 版权申诉】”(推荐),意见反馈和侵权处理邮箱:1219186828@qq.com;也可以拔打客服电话:4008-655-100;投诉/维权电话:4009-655-100。