生物医学光子学与激光散斑成像科学探究.pptx
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1、Lab of biomedical photonicshttp:/Laser Speckle Contrast ImagingTheory,Instrumentation and ApplicationsDepartment of Biomedical EngineeringLab of Biomedical PhotonicsBiomedical Optics TopicLab of biomedical photonicshttp:/02 May 2024Page 2Introduction to Biomedical Optics Diffuse Optical Tomography(D
2、OT)Photoacoustic Imaging(PAI)Multi-spectral Optical Intrinsic Signal Imaging Molecular Imaging Ultrafast Photography High Resolution Altas of Mouse Brain Laser Speckle Contrast Imaging Theory Instrumentation ApplicationsOulineLab of biomedical photonicshttp:/02 May 2024Page 3General optics theoryThe
3、rapeutic applications of lightDiagnostic optical methods Medical optical imaging is the use of light as an investigational imaging technique for medical applications.Biomedical OpticsLab of biomedical photonicshttp:/02 May 2024Page 4Diffuse Optical Tomography(DOT)Nat Photonics.2014 June;8(6):Lab of
4、biomedical photonicshttp:/02 May 2024Page 5Diffuse Optical Tomography(DOT)Lab of biomedical photonicshttp:/02 May 2024Page 6Photoacoustic Imaging(PAI)Lab of biomedical photonicshttp:/02 May 2024Page 7Photoacoustic Imaging(PAI)Lab of biomedical photonicshttp:/02 May 2024Page 8Photoacoustic Imaging(PA
5、I)Lab of biomedical photonicshttp:/02 May 2024Page 9Multi-spectral Optical Intrinsic Signal ImagingLab of biomedical photonicshttp:/02 May 2024Page 10Multi-spectral Optical Intrinsic Signal ImagingSimultaneous detection of hemodynamics,mitochondrial metabolism and light scattering changes during cor
6、tical spreading depression in rats based on multi-sNeuroImageVolume 76,1 August 2013,Pages 7080Lab of biomedical photonicshttp:/02 May 2024Page 11Molecular imagingMarina V Backer,NATURE MEDICINE 2007,13Lab of biomedical photonicshttp:/02 May 2024Page 12Molecular imagingW.T.Li,BIOMEDICAL OPTICS EXPRE
7、SS,2014,2679L.M.Nie,ACS Nano,2014Lab of biomedical photonicshttp:/02 May 2024Page 13Molecular imagingIn vivo photoacoustic imaging of brain injury and rehabilitation by NIR dye labeled mesenchymal stem cells with enhanced brain barrier permeabilityWeitaoLi,#,HongkeWang,#,JingLv,#,RongheChen,YuLiu,Ya
8、Peng,andLimingNie,*Lab of biomedical photonicshttp:/02 May 2024Page 14Ultrafast Photography L Gao et al.Nature 516,74-77(2014)Lab of biomedical photonicshttp:/02 May 2024Page 15Ultrafast Photography high speed digital image sequenceLab of biomedical photonicshttp:/02 May 2024Page 16High Resolution A
9、ltas of Mouse BrainLab of biomedical photonicshttp:/02 May 2024Page 17High Resolution Altas of Mouse Brain Li A,2010,Science,330(6009)Lab of biomedical photonicshttp:/02 May 2024Page 18Laser Speckle Contrast Imaging Theory Instrumentation ApplicationsOulineLab of biomedical photonicshttp:/02 May 202
10、4Page 19What is laser speckle contrast imaging?J.Senarathna,IEEE Reviews in Biomedical Engineering,6,2013Lab of biomedical photonicshttp:/02 May 2024Page 20When coherent light scatters from a random medium,the scattered light produces a random interference pattern called speckle.Speckle Definition*D
11、.A.Boas,Journal of Biomedical Optics,2010,15(1)#D.Briers,Journal of Biomedical Optics,2013,18(6)Lab of biomedical photonicshttp:/02 May 2024Page 21SpeckleJ.Senarathna,IEEE REVIEWS IN BIOMEDICAL ENGINEERING,6,2013Lab of biomedical photonicshttp:/02 May 2024Page 22The theoretical basis for analyzing s
12、peckle intensity fluctuations dates back to the late 1960s with the development of dynamic light scattering.History-1960R.Pecora,Annual Review of Biophysics and Bioengineering,1972Lab of biomedical photonicshttp:/02 May 2024Page 23There was extensive activity in the 1970s rigorously relating speckle
13、 temporal dynamics to various forms of particle dynamics in dilute single-scattering suspensions.History-1970In vivo evaluation of microcirculation by coherent light scatteringNature 254,56-58(06 March 1975)Lab of biomedical photonicshttp:/02 May 2024Page 24A solution was presented in the 1980s usin
14、g a camera to obtain a quick snapshot image of a time-integrated speckle pattern,History-1980A.Fercher,Optics Communications 37(5),1981Lab of biomedical photonicshttp:/02 May 2024Page 25This method,laser speckle contrast imaging(LSCI),was advanced in the 1990s for imaging blood flow in the retina an
15、d skin with the availability of faster digital acquisition and processing technologies.History-1990Lab of biomedical photonicshttp:/02 May 2024Page 26The speckle image of 2-D maps of blood flow can be obtained with very high spatial and temporal resolution by using a CCD camera and quantifying the s
16、patial blurring of the speckle pattern.To quantify the blurring of the speckles,the speckle contrast,defined as the ratio of the standard deviation to the mean intensity,is computed as,Theoretical BackgroundNote that we use s to refer to the spatial standard deviation of the speckle intensity,K is t
17、he spatial speckle contrast,and is the average intensity of speckle image.Lab of biomedical photonicshttp:/02 May 2024Page 27激光散斑衬比成像基本原理Lab of biomedical photonicshttp:/02 May 2024Page 28This gives a speckle contrastTheoretical BackgroundLab of biomedical photonicshttp:/02 May 2024Page 29Modulate o
18、f LSCI(a)Spatial Speckle Contrast(b)Temporal Speckle Contrast(c)Spatial versus Temporal Speckle ContrastLab of biomedical photonicshttp:/02 May 2024Page 30激光散斑空间衬比分析如图所示,图中的每个方格代表一个 CCD图像上的像素点,第一个 NsNs 的方框中的像素点的衬比值赋给中央点,方框右移一个像素点。如此循环,以此对所有区域进行同样的运算,最后获得各个区域的衬比值。计算空间衬比的流程示意图Lab of biomedical photoni
19、cshttp:/02 May 2024Page 31激光散斑时间衬比分析计算时间衬比的流程示意图如图所示,对这一系列图片空间上每一个像素都对应有一个光强变化的时间序列,对这一个时间序列进行时间衬比统计,则获得该点的时间衬比,类似于对空间上每一个像素点做类似处理。Lab of biomedical photonicshttp:/02 May 2024Page 32激光散斑空间衬比分析计算时空衬比的流程示意图基于时间和空间的衬比分析方法,衍生出多种将空间与时间统计相结合的衬比分析方法。总体思路如图所示,对NsNsNt个像素统计Lab of biomedical photonicshttp:/02
20、May 2024Page 33激光散斑衬比分析方法空间衬比K值图时间衬比K值图时空衬比K值图Lab of biomedical photonicshttp:/02 May 2024Page 34Instrumentationllaser light in the wavelength range of 600 nm to 900 nmla 10 bit or higher resolution CCD camera:exposure times may range from 1 ms to 20 ms;frame rates in the order of 10 to 30 frames pe
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- 关 键 词:
- 生物医学 光子 激光 成像 科学 探究
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